Additional Cell Culture Reagents and Supplements
Advanced Biomatrix Poly-D-Lysine, Solution, 0.1 mg/mL
Poly-D-Lysine comes with 50 mL of a 0.1 mg/ml sterile-filtered solution. Poly-D-Lysine is a synthetic amino acid chain that is positively charged and widely used as a coating to enhance cell attachment and adhesion to both plasticware and glass surfaces. This molecule is resistant to enzymatic degradation and has been used to culture a wide variety of cell types. The molecular weight of Poly-D-Lysine can vary significantly with lower molecular weight (30,000 Da) being less viscous and higher molecular weight (>300,000 Da) having more binding sites per molecule. This product’s molecular weight ranges from 70,000 to 150,000 Da yielding a solution viscosity for easy handling while providing sufficient binding sites for cell attachment. Poly-D-Lysine surface coatings are designed to improve cell attachment, growth and differentiation of many cell types. Coated surfaces will often improve cell attachment in reduced or serum-free conditions.
Genscript Corporation Laminin 521 Human
Laminin 521 composed of alpha 5 beta 2 and gamma 1 chains is a reliable matrix for feeder-free culture of human embryonic (hES) and induced pluripotent (hiPS) stem cells When combined with defined maintenance media Laminin 521 consistently supports self-renewal of human hES and hiPS cells without spontaneous differentiation
Sigma Aldrich Fine Chemicals Biosciences IL 4 human Animal componen
IL-4 is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation survival and gene expression. Produced by mast cells T cells and bone marrow stromal cells IL-4 regulates the differentiation of naive CD4 T cells into helper Th2 cells characterized by their cytokine-secretion profile that includes secretion of IL-4 IL-5 IL-6 IL-10 and IL-13 which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergy. Recombinant human IL-4 is a 14.9 kDa globular protein containing 129 amino acid residues.
Quality Biological Inc M9 CA Medium pH 7.1 500mL
M9 CA Media contains the essentials for bacterial species to grow. Important components of M9 CA medium are the carbon source from glucose a nitrogen source from ammonia chloride and electrolytes from sodium chloride potassium calcium phosphorus and magnesium salts in the M9 solution.Glucose as a carbon source and Casamino acids as a nitrogen source. Manufactured in an ISO 90012015 certified facility.
STEMCELL Technologies STEMdiff Monocyte Differentiation Supplement (100X), Size: 1 mL
Use STEMdiff Monocyte Differentiation Supplement (100X) to support the differention of human pluripotent stem cells (hPSCs) to CD14sup/sup monocytes. Available either individually or as part of the STEMdiff Monocyte Kit (/products/stemdiff-monocyte-kit.html) the supplement is intended to be combined with StemSpan SFEM II (/products/stemspan-sfem-ii.html) to prepare the Monocyte Differentiation Medium. This medium can be used during the STEMdiff monocyte differentiation protocol to promote the production of CD14sup/sup monocytes.
Zymo Research Corporation Expansion Broth (EB), 100 ml
Uniquely formulated Expansion Broth™ (EB™) that is used in conjunction with our Overexpression Broth™ (OB™) for consistent high level recombinant protein expression.
ILEX LIFE SCIENCES LLC 50X NEUROPAN-27 100 ML
PAN-Biotech Neuropan-27 Supplement (50x): Defined Serum Substitute for Serum-Free Culture of CNS Neuronal Cells (100 ml). DESCRIPTION: Neuropan-27 is provided as a 50x concentrated serum substitute designed for the long-term growth and survival of hippocampal and other neuronal cells of the Central Nervous System (CNS). By supplementing Neuropan Basal Medium with Neuropan-27 and 0.5 mM L-Glutamine, excellent long-term viability of rat embryonic hippocampal neurons has been achieved after several weeks in culture. Glial cell growth is reduced to less than 0.1% for a largely pure neuronal cell culture. COMPOSTION: Neuropan-27 Supplement is a serum substitute of defined composition containing albumin, insulin, transferrin, lipids, and hormones. It is used as a supplement to Neuropan Basal Medium to support growth and survival of neuronal cells from the CNS. LIQUID/POWDER: Liquid. STERILE: Yes. STORAGE TEMP: -20°C. MANUFACTURER: PAN-Biotech GmbH (Germany).
Sigma Aldrich Fine Chemicals Biosciences TrypZean bovine recombinant, expressed in corn, lyophilized powder, ≥3,350 units/mg solid (USP) | 9002-07-7 | MFCD04041188 | 10mg
TrypZean bovine recombinant, expressed in corn, lyophilized powder, ≥3,350 units/mg solid (USP) | Mol Wt: | 9002-07-7 | MFCD04041188 | 10mg
Crescent Chemical Co Inc TYLOSINE-TARTRATE SOLN (100X)
Tylosine-tartrate solution (100x) sterile filtered Macrocyclic antibiotic with large lactone ring. Inhibits bacterial protein synthesis at the ribosomal 50S-subunit. Acts bacteriostatic, in higher concentrations as well bactericidal. Active against Gram-positive bacteria and mycop
Research Products International Corp Gelrite, 100 Grams
Naturally derived gelling polymer that can be used in place of agar. Gelrite forms clear, rigid gels at approximately half the use level of agar in presence of soluble salts like Mg2+ and Ca2+.
Sigma Aldrich Fine Chemicals Biosciences Albumin from human serum lyophilized powder, essentially globulin free, >=99% (agarose gel electrophoresis) | 70024-90-7 | MFCD00081418 | 250MG
Albumin from human serum lyophilized powder, essentially globulin free, >=99% (agarose gel electrophoresis) | Purity: >=99% (agarose gel electrophoresis) | 70024-90-7 | MFCD00081418 | 250MG